The
eggs retrieved from the follicular fluid is kept in a CO2 incubator for 4-10 hrs for maturation and then
50-100 thousand processed sperm are placed around each egg . In some cases
where sperm count are very low, the process of fertilization must be assisted
via the injection of individual sperm into each egg, process called ICSI. There
are few techniques to process semen and to take highly motile sperm for
fertilization. Techniques like “swim-up” technique density gradient and Ficoll
entrapment are used to prepare sperm for insemination.
ICSI [Intra Cytoplasmic Sperm Injection]:
Intra Cytoplasmic Sperm Injection (ICSI)
ICSI [Intra Cytoplasmic Sperm Injection]:
Intra Cytoplasmic Sperm Injection (ICSI)
Either
Male or female factor where the IVF is not successful, with the help of a
special instrument called a micro manipulator, an ICSI is done
Indication
for ICSI
·
Low sperm count
o
Unexplained failure or poor fertilization in
previous IVF cycles.
o
Severe structural abnormalities of sperm.
o
Immotile sperm. Eg. Inherited disorders of
the sperm tail for which no other IVF technique is effective (ex: Kartagener's
Syndrome).
o
Epididymal
or testicular sperm obtained from Azoo spermic patients (No sperm count
in semen, but normal or low sperm production in the testis)
o
Sperm obtained by electro ejaculation or from
urine incases of retrograde ejaculation.
o
HIV / AIDS, Hepatitis A, B, C affected male
partner.
Steps
of ICSI :
Eggs
are first retrieved and placed in a tiny drop in a special dish. While viewing
the egg and sperm through a microscope, a single sperm is captured in a fine
glass needle and injected through the egg shell and into the center of the egg.
Successful fertilization is evaluated the following day. Three to five days
after ICSI embryos are transferred into woman's uterus. ICSI treatment India ,
Cost ivf india
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